CHARACTERIZING BREAST CANCER INVASION AND PROLIFERATION WHEN CO-AGGREGATED WITH ADIPOCYTES IN MULTICELLULAR…

Assistance award details

Assistance award identifier

ASST_NON_SC3GM144192_075

FAIN

SC3GM144192

Recipient

CALIFORNIA STATE UNIVERSITY SAN MARCOS CORP

Awarding agency

Department of Health and Human Services

Funding agency

Department of Health and Human Services

Assistance listing (CFDA)

93.859

Total obligated

$447,000

Period

Jun 1, 2022 – May 31, 2026

Fiscal year

2022

Place of performance state

CA

Source file

FY2022_All_Assistance_Full_20261006.zip

Description

CHARACTERIZING BREAST CANCER INVASION AND PROLIFERATION WHEN CO-AGGREGATED WITH ADIPOCYTES IN MULTICELLULAR SPHEROIDS CREATED WITH A CUSTOM BIOREACTOR TO AUGMENT CELL-CELL CONNECTIVITY. - PROJECT SUMMARY BREAST CANCER IS THE MOST COMMON FORM OF CANCER IN WOMEN AND IS CONSIDERED A MODERN-DAY EPIDEMIC, ESPECIALLY BECAUSE OF THE HIGH DEGREE OF HETEROGENEITY. MULTIPLE STUDIES HAVE SHOWN THAT THE TUMOR MICROENVIRONMENT CONTRIBUTES TO CANCER GROWTH AND CHEMORESISTANCE. CANCER-ADIPOCYTE INTERACTIONS HAVE GAINED INCREASED ATTENTION AS THE CROSSTALK BETWEEN THEM HAS BEEN SHOWN TO PROMOTE TUMOR PROLIFERATION AND INCREASED CANCER MIGRATION. HOWEVER, THE PHYSIOLOGICAL RELEVANCE OF PREVIOUS CO-CULTURE METHODS IS QUESTIONABLE. SEVERAL PAST STUDIES DID NOT USE HUMAN CELLS, OR DIFFERENTIATE BETWEEN WHITE AND BROWN ADIPOCYTES, WHICH HAVE DIFFERENT PHENOTYPES. IN THIS PROPOSAL WE WILL ANALYZE TUMOR INVASIVENESS OF HUMAN TRIPLE NEGATIVE AND LUMINAL A BREAST CANCER CELLS WHEN CO-CULTURED WITH HUMAN BROWN AND WHITE ADIPOCYTES. TO DO THIS, WE PROPOSE TO USE A 3D MULTICELLULAR TUMOR SPHEROID SYSTEM. TUMOR SPHEROIDS PROVIDE A PHYSIOLOGICAL RELEVANT ENVIRONMENT TO STUDY CANCER BIOLOGY AND DRUG DISCOVERY. COMPARED TO 2D PARADIGMS, RESEARCH SHOWS 3D MODELS BETTER RECAPITULATE CHEMORESISTANCE AND TUMOR GROWTH FOUND IN HUMAN IN VIVO TUMORS. IN THE LAB, WE HAVE DEVELOPED A BIOREACTOR THAT USES SURFACE VIBRATIONS TO PRODUCE FARADAY WAVES THAT AGGREGATE CELLS INSIDE A LIQUID CHAMBER. DEVICES WITH SIMILAR TECHNOLOGY HAVE BEEN USED TO ASSEMBLE SCAFFOLD-FREE 3D CELL CONSTRUCTS AND SPHEROIDS IN A RAPID AND SCALABLE WAY, WITHOUT THE NEED FOR ADDITIONAL CHEMICALS OR INTRUSIVE PROCEDURES. IN THIS GRANT, WE ALSO SEEK TO DETERMINE IF THIS TECHNOLOGY GENERATES MULTICELLULAR SPHEROIDS WITH GREATER CELL-CELL ADHESION, COMPARED TO STANDARD SELF-ASSEMBLY. TO ACHIEVE THE SCIENTIFIC AND TECHNOLOGICAL GOALS OF THIS GRANT, TWO SPECIFIC AIMS ARE PROPOSED: THE FIRST AIM WILL COMPARE THE VIABILITY AND CONNECTIVITY OF