Obtaining High-Accuracy Consensus Sequences via Flossing

Project Summary While general DNA sequencing is becoming a standard laboratory techniqueno technology to date has been able to combine the features of high accuracyandgtand long readss tos of kilobasesan absolute necessity to broadly transition DNA sequencing into a practice for genomic and precision medicine with diagnostic applicationsWhile single molecule sequencesSMSapproaches have emerged as very promising toward overcoming read length limitations posed by next generation sequencingNGStechnologiesit is unfortunately commonplace for these SMS technologies to yield error rates that exceedand higher accuracy is needed to obtain medically relevant sensitivity specificity metricsDuring this programElectronic BioSciencesEBSproposes to demonstrate high accuracyandgtSMS via electronic strandflossingwithout using processive enzymeswhich will lay the foundation for the eventual development of the first high accuracylongread SMS systemHereEBS will demonstrate high accuracysingle molecule consensus sequence calling using iterative reads on individual DNA strandsThe accomplishments made during the program will enable exciting progress within the sequencing fieldincluding fundamental investigations into genome assemblysampling profilinggeneral biological studiese gtelomer lengthmicrosatellite sequencingetcand eventually clinical diagnosticsfeats that are otherwise challenging to perform with currently available technologyAs a resultthis work will help improve broad scale biodiversity and metagenomic studiesclinical diagnosticsand population care Project Narrative Developing tools that enable rapid and inexpensive single molecule sequencingSMSwith both high accuracy and long read capabilitieshas the potential to revolutionize numerous fields including medicine and clinical diagnosticsforensicsenvironmental investigationsde novo genome assemblywidespread population genetics investigationsand general biological studiese gtelomer lengthmicrosatellite sequencingetcWith specific regard to medical applicationssuch advancements will enable routine genomic and precision medicine for comprehensive and personalized patient screeningpredictive risk assessment and treatmentthus leading to substantial improvements in population healthcare and survival