In-Situ Cryopreservation Without Cryoprotectants

SUMMARY With recent rapid advances in biomedical technologiesastronomical numbers of cell lines are emerging as highly valuable and promising products for therapies or critical research toolsThe ability to preserve stocks of quality controlled cells on both industrial and laboratory scales is an important challengedue to numerous technical and practical issues and regulatory concerns associated with traditional cryopreservation approach that requires the use of cryoprotectantsThe present proposal specifically addresses this challenge by developing of a novelin situcryopreservation technology that doesn t require the use of any cryoprotectantOur preliminary data demonstrated that efficacy of our novel ultra fast cooling approach to successful cryopreservation of partial corneal endothelia without using any cryoprotectantThe over goal of this proposal is consequently to produce and test the prototype of a device system that enables in situ cryopreservation of cell suspensions with the volumes sufficient for practices in both biomedical research and clinical applicationsHuman adipose derived mesenchymal stem cellshASCwill be used as the model for in vitro studies and the design and production of the device prototype will be optimized through an iterative bioengineering approachThis Phase I study paves the path for future engagement of the proposed methodPhase IIin enhancing the efficacy of using hASC and other valuable cell types for cell therapy and tissue engineering by potentially improving the quality of cell lots and minimizing donor to donor variability NARRATIVE Storage of cell lines with reliable quality and efficiency is of critical importance for biological research and biomedical applications meeting an ever increasing clinical needHowevertraditional cryopreservation approach using cryoprotectants incurs significant donor to donor variabilityregulatory concernsand complicated and inefficient proceduresTo overcome these challengesthe overall goal of the present proposal is to innovate cell cryopreservation technology by developing a novel method asin situcryopreservation of cell suspensions without using any cryoprotectant and with significantly improved performance efficiency and reliability