Noninvasive multiphoton imaging of subcellular structures with color contrast for rapid detection of skin cancers

Nonmelanoma skin cancer NMSC represents the most common form of cancer in the human body The method for diagnosing and treating NMSCs requires removal of a biopsy that is prepared and stained for analysis on a standard optical microscope This process is painful for patients introduces a substantial delay into the flow of care and represents a considerable cost to healthcare $ B yr due to the large number of unnecessary biopsies Zebra Medical Technologies ZMT aims to address the need for a better method to diagnose NMSCs by creating a noninvasive imaging technology capable of displaying histology like images of skin cellular structure in real time In this project ZMT will construct a portable skin imaging microscope for human clinical studies Aim develop software for data acquisition image processing and improved user interface Aim and demonstrate in human subjects with basal cell carcinoma BCC the ability to detect pathologic features of BCC Aim ZMT is the creator of the only commercially available fiber coupled multiphoton microscopy MPM system for in vivo imaging of skeletal muscle cellular structures and contractile dynamics the Zebrascope which has been sold to three universities and one hospital to date ZMT will adapt and optimize technologies invented for the Zebrascope to specifically visualize cellular structures in skin The ZMT team possesses expertise in miniaturizing optical systems designing disposable optical probes with highly repeatable alignment imaging deep into living tissue and conducting human studies with MPM under IRB oversight ZMT has already proven feasibility of this concept with a bench top prototype that successfully imaged subcellular structures in the epidermis and dermis in living human skin and in excised tumor samples Success in this project will lead to a larger clinical study in Phase II ZMT views NMSC as a natural first target for real time diagnosis that paves the way for applications in melanoma diagnosis followed by other epithelial cancers such as oral esophageal and cervical Zebra Medical Technologies is developing a noninvasive microscopy system to provide images of suspected nonmelanoma skin cancer with subcellular resolution to aid in the diagnosis and treatment of this most common form of cancer This technology will reduce the number of patient incisions increase the speed and efficiency of diagnosis and treatment and reduce the cost of unnecessary biopsies