A rapid, culture-free whole genome approach for characterizing complex metagenomics samples

Project SummaryAbstractDovetailGenomicshasadaptedourinvitroproxityligationmethodforfecalmetagenomicsapplicationsWearedevelopingapowerfulandefficientapproachtodenovometagenomicsassembly that will allow the research and biomedical communities to move beyond single locusmolecule counting or statistical inferenceSuccessful development of this approach will provideasingleintegratedworkflowforaccurateassemblyofallmajorconstituentsofcomplexmetagenomicscommunitiesThistoolwillenableacomprehensiveunderstandingofthewaysour gut microbiome influences human health and diseaseInthisprojectDovetailGenomicswilldevelopthemodificationstothelaboratoryproceduresnecessary to efficiently capture and represent the diversity of microbes present in a human fecalsampleWewillalsomodifyanddevelopanewcomputationalapproachtometagenomicsassemblythatexploitstherichdatatypethatwegenerateThiscomputationalapproachwillachieve highly contiguous scaffolding and strain deconvolutionWe envision a robustfoolprooflaboratory protocol and software product that allows a clinician to generate a comprehensive viewof the dynamic human gut microbial environment from a small fecal sample in a manner of days