A Comprehensive 5K plus Glycan Microarray

Project SummaryAbstract There is growing need of high throughput analytical platforms to study interactions of cell surface glycans and glycan binding proteinsWe propose here to develop aKglycan array to facilitate functional glycomics researchTwo hurdles for development of a comprehensive glycan array arelack of a sensitive glycan array substrate enabling multivalent binding eventsandlack of a comprehensive glycan library of glycan structures representing the whole glycomesTo solve this challengeZ Biotech will team with Chemily Glycoscience to deliver the most comprehensive glycan array and glycan sub arrays to marketZ Biotech invented a robust microarray substrate having the advantage to display glycans on solid surface in multivalent formand Chemily is a leading glycoscience company in production of complex glycanssugar nucleotides and glycan related enzymesThe proposed research strategy has been supported by a preliminary experimental investigation under collaboration between the two companiesIn Phase Iour specific aims arecollection and synthesis ofKglycans incategoriessub arraysandKglycan array manufacturing and QC QA evaluationFinallythe glycan arrays will be provided to large glycan array screening centers for further evaluationThese are realistic goals within a SBIR program frame and the resultant glycan array products will have a broad impact on glycobiology researchglycan related biomarker characterization and new drug development