A fluorescent reporter detecting precise homologous recombination transgenesis activity

The broadlong term objective and specific aims of the fast track SBIR research are to create areagentRecombiREDfor use in microinjection of embryos for identifying a subset of embryosas recombination competent injectionsThe ability to quickly identify genome editing events inan embryo allows researcher to quickly make precision animal models of human disease statesPrecise animal models allow for greater scientific understanding of a human disease andprovides a robust platform for drug discovery screeningThe RecombiRED reagent relies oninduced fluorescence in response to activation of homologous recombination repair within theembryoDetection of homologous recombination repair in injected embryos with RecombiREDallows animal husbandry and further screening efforts to be restricted to a subset of embryos byup tox for having a desired precise knockin of new genetic material at a specific geneticlocusThe phase I research adapts a the RecombiRED reagent developed in Celegans modelorganism to have the appropriate expression control elements enabling sufficient activity inzebrafish embryoIn Phase IIthe additional research further optimizes parameters of theRecombiRED reagent to minimize false positive and false negative readoutswhich then allowsmaximal forecasting of targeted genome edit eventsFinallya set ofreal world transgenesisprojects are used as premarket beta test to demonstrate the ubiquity and robustness of theRecombiRED for simplifying discovery of genome editing in the creation of animals models foruse in drug discoveryThe relevance of this research to public health is the creation of an experimental tool forsimplifying the discovery of genetically altered model organismsThe tool allows faster creationof animal models for use in better scientific discovery and faster drug development