Wireless Technology for Long Term Non Invasive O Sensing within Cell Encapsulation Devices in Vivo

Summary Islet cell transplantation is an alternative therapy for the treatment of diabetes but requires the administration of immunosuppressive drugs to avoid host rejection Immunoisolation devices such as TheraCyte device protect islet cells from the host response and thus eliminate the need for immunosuppressive drugs However once implanted hypoxic conditions around and within the device result in loss of islet cell function and viability To alleviate these conditions the Papas lab in collaboration with TheraCyte and other industry partners developed devices that enable supplemental oxygen delivery to encapsulated cells Monitoring oxygen supply within implanted devices is now recognized as critical but it is limited by currently available instrumentation that is bulky invasive or have slow measurement times The Lumee O sensor by Profusa is a flexible wireless injectable fluorescent sensor that provides continuous and non invasive measurements of oxygen The Lumee O sensor has been tested extensively in small and large pre clinical models as well as in humans for up to year We propose the integration of Profusa Lumee O sensors into oxygen enabled TheraCyte immunoisolation devices for the non invasive monitoring of oxygen concentrations within these devices Specific aims for Phase I will focus on in vitro validation of oxygen measurements from Lumee O sensors within oxygen enabled TheraCyte devices and assess different oxygen supplementation approaches as measured by Lumee O sensors on device vascularization in vivo Main outcomes of this collaborative effort will be monitoring oxygen delivery to devices to improve islet cellular function and the demonstration of Lumee O sensors as a new tool for oxygen monitoring within implantable cellular based devices Narrative Immunoisolation devices for islet cell transplantation require oxygen supplementation to maintain cell function and viability in the hypoxic tissue environment post implantation We propose to integrate Profusa s Lumee O sensors into oxygen enabled TheraCyte immunisolation devices as a method for non invasively monitoring oxygen delivery and concentration within these devices Successful integration of these devices will allow for better monitoring of oxygen levels and supplementation and understanding the effect of oxygen concentration on device function