Tools to Measure Non Receptor Tyrosine Kinase Activities for Drug Discovery and Biomarker Analysis

Project Abstract Advanced cell based technologies for kinase activity profiling are needed for earlier identification of next generation kinase inhibitors and to enable clinical evaluation of kinase activity biomarkers The long term goal of this proposal is to generate targeted kinase activity tests compatible with viable cells from patient derived samples The objectives in this proposal work toward that goal by developing prototype assays that measure Jak and Src family SFK kinase activity specifically in disease relevant cellular models Peptide based substrates phosphorylated specifically by Jak and SFK have been validated in vitro In this proposal they will be adapted for cell based use and it will be shown that intracellular phosphorylation of the substrates is specifically driven by the kinase of interest In addition the assays will be analytically characterized and used in screening applications relevant to drug discovery Successful completion of these prototype assays will validate an Randamp D pipeline for developing additional cell based targeted kinase activity assays In Phase II cell based assays for additional kinases will be developed partnerships for commercialization in the drug discovery kinase profiling market will be formalized and compatibility with viable cells derived from patient samples will be evaluated Narrative This proposal pushes toward the goals of precision medicine by overcoming technical hurdles to measure endogenous kinase activity in cell based application Kinases are an important class of drug target in oncology with over dozen kinase inhibitors already FDA approved and hundreds more in pre clinical and clinical development The target assays developed in this proposal have applications in drug discovery for identifying new kinase inhibitors and have potential to be translated into clinical applications for personalized characterization of kinase activity markers For clinical application this information can act to stratify patients into responders and non responders for kinase inhibitors alleviating the clinical and economic burden presented by heterogeneous patient response Accomplishing the milestones in this proposal will result in turnkey tools for drug discovery that allow researchers to better evaluate potential compounds by providing assays compatible with disease relevant cellular models