Point-of-Care Nitric Oxide Sensor for Wound Management

DESCRIPTION provided by applicant This Small Business Innovation Research SBIR Phase I project aims to develop a microfluidic based nitric oxide NO sensor to prospectively identify wounds that are unlikely to heal Each year over million Americans develop chronic wounds such as pressure ulcers and diabetic foot ulcers In the U S alone the high prevalence of chronic wounds is a $ billion economic burden Currently clinicians rely on medical history physical examination and wound imaging to assess healing progress A point of care device that rapidly identifies a specific biochemical cause for poor healing would allow clinicians to enact proper therapies immediately Nitric oxide NO is a key mediator in all stages of wound healing As would be expected wounds deficient in NO heal poorly and treatments that restore NO bioactivity are able to accelerate wound healing Recent clinical studies have shown that nitrite concentrations in wound fluid i e a stable end product of NO synthesis are able to indicate wounds that are less likely to heal While promising nitrite accumulates over time and only provides a andquot historyandquot of the wound giving no indication of real time NO production that would reflect the current healing trajectory of the wound nor enabling repeat measurements that could monitor the effectiveness of new treatments Until recently direct detection of NO has been tedious expensive or sample volume intensive Clinical Sensors Inc has developed a microfluidic electrochemical sensor that rapidly and selectively detects NO directly in andlt L volumes with nanomolar limits of detection In this proposal we aim to assess our sensorandapos s ability to measure NO in simulated wound fluids and validate the sensorandapos s accuracy After completing this work we will be well positioned to further develop this product through animal wound models and clinical studies Once fully developed our sensor technology will enable clinicians to quickly assess wound status at the point of care select appropriate therapies and monitor the effectiveness of those therapies over time Ultimately our sensor may enable clinicians to quickly assess wound status choose appropriate treatment plans during patient assessment i e point of care and monitor the effectiveness of those treatments over time PUBLIC HEALTH RELEVANCE Chronic wounds such as pressure ulcers and diabetic foot ulcers reduce the quality of life for sufferers and place up to a $ billion dollar burden on the healthcare system each year Identifying wounds that are unlikely to heal is a key step in wound management The objective of this project is to develop a lab on a chip device that allows for rapid detection of nitric oxide to facilitate early diagnosis and monitoring of non healing wounds reduce hospital costs and save lives