Semiconducting Polymer Dots for Multiplexed Assays

DESCRIPTION provided by applicant Fluorescence based methods play an essential role in modern cell biology and biomedical research Further development of these techniques has been hindered by the lack of suitable fluorescent probes To overcome this challenge various fluorescent nanoparticles have been developed Among those semiconducting polymer dots Pdots exhibit superior properties such as high brightness fast emission rate and excellent photo stability Fluorescence based techniques encompass a broad area with a large market size ranging from immuno assays to super resolution imaging And while Pdot has proven to be an excellent general fluorescent probe to maintain focus to penetrate the market and to generate adoption by the biomedical community we have decided to go after flow cytometry applications owing to its large market potential During our month Phase I SBIR project we have developed Pdot and Pdot with ultra high brightness narrow band emission and high photo stability To achieve this goal of penetrating the flow cytometry market this Phase II SBIR project proposes to develop additional Pdot probes to complement the developed during Phase I After this Phase II project we will have completed a set of Pdots so we may launch a Pdot series with high brightness and narrow emissions for flow cytometry applications Furthermore we will establish pre production and quality control protocols for manufacturing this Pdot series PUBLIC HEALTH RELEVANCE Fluorescence based techniques play an essential role in modern cell biology and biomedical research as well as in various clinical diagnostic assays This proposal aims to develop a new class of fluorescent probes that should offer significant performance improvements over traditional fluorescent dyes and nanoparticles When successfully developed this new probe will find broad use in biomedical research and clinical diagnostics