Tools for drug efficacy studies in perfused tissue models

DESCRIPTION provided by applicant Organ on a chip tools that recapitulate human biology physiology and pathology are critically needed in basic and applied science research toxicology and drug lead development to ultimately extend and improve the quality of life by advancing knowledge and expediting discovery while reducing research costs and animal sacrifice Unfortunately most products incorporating circulation mimicking perfusion are too complicated for researchers to use incompatible with many protocols and reads and low throughput Our goal is to change this for ordinary lab personnel and for researchers across disciplines We will make a researcher centric product which does not require special skills or training for use a tool that is flexible enough to serve diverse research objectives and a tool which for pharmaceutical industry means a simple low cost platform for more predictive multiplexed testing of drugs and drug combination strategies Lena Biosciencesandapos organs on a chip will comprise artificial vasculature and be in a standard screening accessible format known to any user in life sciences biotechnology and drug discovery for ease of use and user adoption This platform will support vascularized D organ models for physiologically closer drug delivery and distribution by mimicking vasculature to tissue resistance and intra tissue resistance to drug transport It will be especially well suited for in vitro testing of humanized high molecular weight therapeutics which are administered to patients intravenously and retained in circulation for the period of weeks The platform will further provide concentrated cel secretome proteome metabolome degradome interactome etc to facilitate detection and identification of biomarkers of therapeutic efficacy cellular and biochemical changes in response to drugs and other stimuli and therefore serve as a diagnostic and prognostic research tool in order to for example prevent serious reactions in clinical trials Next this organ on a chip tool will also lend itself useful for development of de novo drug resistant tissue clones The acquired drug resistance tissue clones will enable testing of new drug combinations that are critically needed for approved frontline therapy drugs on which patients eventually relapse Lastly this platform will be in sufficient throughput compliant with most protocols assays reads and imaging setups researchers use routinely and have zero dead volume to minimize use of expensive developmental drugs The platform utility will be demonstrated using D models of liver brain and breast cancer and further validated by demonstrating in vitro testing of drugs and their combinations PUBLIC HEALTH RELEVANCE This vascularized organ on a chip research platform will enable tissue fingerprinting and drug testing in vitro in perfused organ models It will advance public health by enabling faster low cost testing of drug candidates for the ultimate safe and effective treatment of cancer inflammatory infectious autoimmune neurological and cardiovascular diseases