High Spatial Resolution Ultrafast Spectroscopy

Chemical sensitive imaging of heterogeneous materials and biological cells requires a sensitive, high spatial resolution microscopy method based on vibrational resonances that is capable of performing in situ spectroscopy with both frequency and time resolution. Statement of how this problem or situation is being addressed. In this SBIR/STTR, we will develop a new coherent Raman microscopy technique that will improve sensitivity over existing stimulated Raman microscopy techniques by up to three orders of magnitude or more, and will allow systematic background removal without requiring reference spectra. It will provide precision in spectral and temporal resolution, and have high chemical sensitivity with spatial resolution approaching 100 nm and femtosecond temporal resolution. What is to be done in Phase I? In Phase I, we will test our proposed technique for sensitivity. Noise levels will be examined, and detection levels checked against known chemical mixtures. We will examine additional design modalities for the technology, and image test samples for chemical content. Commercial Applications and Other Benefits. Our improved coherent Raman microscopy instrument will significantly transform the microscopy market with cell biology, biomedical, energy materials, and environmental science applications. Improvements will have applications in medicine, Homeland Security, and nano- and meta-materials.