Development of an Assay Platform for Measuring Endogenous Kinase Activity

DESCRIPTION provided by applicant Kinase inhibitors are an effective class of targeted therapy for oncology However heterogeneous patient response and acquired resistance continues to be a significant clinical and economic burden To overcome this problem drug developers are pursuing novel kinase inhibitors for existing kinase targets with new modes of inhibition and increased potency as well as compounds for emerging kinase targets There is an unmet need in pre clinical development for assays that measure endogenous kinase activity in disease relevant cellular models The long term goal of this project is to commercialize an assay platform for measuring cell based endogenous kinase activity The goal of this Phase I proposal is to further establish feasibility of an assay platform based on cell permeable peptide substrates specific for a kinase of interest The peptide amino acid sequences are designed using a recently published algorithm developed by Purdue University researchers called KINATEST ID The proof of concept demonstration shows that KINATEST ID designs specific substrates for cytosolic tyrosine kinases based on stringently curated endogenous kinase substrate sequences In this proposal successful completion of the aims will result in the expansion of the platform to show feasibility for receptor tyrosine kinases RTKs In the first ai we will identify endogenous substrates for RTKs in disease relevant cellular models that will be used to design specific substrates for RTKs of interest using KINATEST ID This will be accomplished using a novel method known as kinase assay linked to phosphoproteomics KALIP which identifies endogenous sequences for the target RTK The identified sequences will then be used as input data for the KINATEST ID algorithm to design candidate RTK substrate sequences In the second aim designed substrate sequences will be synthesized and empirically validated for kinase specificity and assay performance will be analytically characterized in vitro Finally substrates that meet quantitative criteria for specificity and assy performance in the second aim will be modified and optimized for use in cell based applications Successful completion of the aims will result in a panel of prototype cell based assays for measuring endogenous RTK activity and a validated Randamp D pipeline for further expansion of the assay platform Phase II of this project will focus on developing partnerships to bring this assay platform to the drug discovery market expansion into additional kinases and adapting the assay for use in patient derived samples such as biopsies or circulating tumor cells PUBLIC HEALTH RELEVANCE The goal of this proposal is to develop a panel of cell based activity assays for receptor tyrosine kinases A secondary outcome of this project will be validation of an Randamp D pipeline for developing additional cell based kinase activity assays Successful completion of the aims will result in prototype assays that can fill an unmet need in drug discovery for measuring endogenous kinase activity in disease relevant cellular models that can be used to improve pre clinical identification of novel kinase inhibitors for oncology