Omics Based Assays for Screening Chemical Toxicity Using Stem Cells

DESCRIPTION provided by applicant There is a paradigm shift in testing and modeling of chemical toxicity Current toxicology calls for cell based vs animal based evidence based and Omics based assays Mesenchymal stem cells MSC play important roles in the maintenance and repair of various tissues including bone cartilage and muscle MSCs are also found in circulating blood and participate in the wound healing process Therefore MSC is arguably one of the most physiologically relevant adult stem cells for chemical toxicology In response to RFA ES Newomics Inc proposes this SBIR Phase II project to complete development of its product high throughput Omics based assays to evaluate the effects of chemical toxicants on proliferation and differentiation of stem cells The Phase II project is buil upon the extremely promising results generated from our Phase I project In Phase I we have demonstrated a robust and universal two stage workflow for Omics based and high throughput screening of chemical toxicity using human MSCs We have also discovered several pathways and networks for mechanistic understanding of chemical toxicity on MSCs In Phase II we will scale up our toxicity assays for MSCs using the Tox k library develop Omics chips and validate their applications in Omics based assays for chemical toxicity and provide a toolkit for screening the effects of chemical toxicants on cell differentiation Once developed our platforms and solutions may be utilized for chemical toxicity studies of other types of stem cells such as ESCs and iPSCs to help prioritize chemicals for in depth toxicological evaluation and to even help develop new intervention strategies for mitigating chemical toxicity thereby contributing to public health PUBLIC HEALTH RELEVANCE Cutting edge technologies enable breakthroughs in biomedical research Developments of mass spectrometry based microchip platforms for screening chemical toxicity using stem cells will help prioritize chemicals for in depth toxicological evaluation thereby providing new strategies for impacting public health