Optimization of Cryoprotectants, Cryotherapeutics, and Protocols for Cryopreservation of Large Tissue Systems

X-Therma Inc. aims to deliver highly effective, non-toxic cryoprotective agents (CPAs) using unique peptidomimetic polymers which not only mimic the outstanding ice inhibiting effects of naturally occurring antifreeze proteins, but also conquer the cons such as high cost and protease degradation. Widely used biopreservation media contain DMSO above cytotoxic concentrations, an unavoidable concern for successful organ/tissue preservation, but remain largely unchanged due to their low cost. Our proposal aims to demonstrate the feasible use of non-toxic, short-chain biomimetic polymers that will manage the growth of ice crystals to sizes compatible with cellular subsistence during cooling and rewarming. X-Therma Inc. benefits significantly as a User Affiliate of Lawrence Berkeley National Laboratory where we have access to state-of-the-art robotic synthesizers and the worlds most renowned authority in this field. We will leverage our distinctive chemical expertise to develop ice controlling biomimetics as commercially favorable additives in cryoprotectant solutions allowing for the substantial reduction of cytotoxic DMSO. Additional funding support will speed the development of a complete cryoprotectant media for advanced organ and tissue preservation allowing cutting-edge surgical techniques for service members and civilians. These chemicals offer non-line of sight industrial applications as ice prevention coatings, hydrating cosmetic additives, and frozen dairy additives.