New Cell Reprogramming Technology to Produce Dopaminergic Neurons

DESCRIPTION provided by applicant Cell Reprogramming andamp Therapeutics LLC will develop new cellular reprogramming technology to produce cell therapeutic product for the treatment of Parkinsonandapos s disease PD Advances in cell reprogramming technologies to generate patient specific cells of a desired type will revolutionize the field of regenerative medicine Over the lat decades several cell reprogramming methods such as nuclear transfer cell fusion and transfection or transduction with pluripotent factors have been developed However the majority of these technologies require the exposure of cell nuclei to reprogramming large molecules via transfection transduction cell fusion or nuclear transfer This raises several technical safety and ethical issues Chemical genetics is an alternative approach for cell reprogramming that uses small cell membrane penetrable substances to regulate multiple cellular processes including cell plasticity The main advantages of this approach over the above mentioned technologies are that the biological effects of small molecules are typically rapid reversible and dose dependent allowing precise control over specific outcomes by fine tuning their concentrations and combinations Recently using chemical genetics approach the combination of small molecules that are involved in the regulation of chromatin structure and function and specific cell signaling pathways we have been able to generate neuronal cells from human mesenchymal stem cells hMSCs that expressed mature dopaminergic DA markers released dopamine exhibited electrophysiological properties of maturing neurons and formed synapses The goal of the proposed Phase I studies is the optimization of this cell reprogramming technology to increase the production of engraftable FOX A TH Nurr midbrain DA neurons and elucidation of the therapeutic effects of these specialized cells in an animal model of PD Phase II studies will focus on clinical grade manufacturing of these DA cells and testing their therapeutic effect in several preclinical pathogenic and etiologic animal models of PD Commercial and clinically compatible research products emerging from Phase I II work include a DA neuron differentiation kit and a technology for large scale clinical grade production of DA neurons The ultimate post Phase II product will be the production of clinically relevant DA neurons for PD therapy To achieve these goals the Phase I aims include Specific Aim will test the hypothesis that the efficiency of DA specification and maturation can be further improved by addition of specific cell signaling modulators and growth factors to our recently developed neural induction protocol at a certain time and in a specific order Immunocytochemistry and RT PCR will be used to evaluate the expression of markers specific to immature and mature dopaminergic neurons Dopamine release will be assessed by ELISA Specific Aim will test the hypothesis that DA cells transplanted into the hydroxydopamine OHDA mice and rat models of PD will survive integrate and will promote restoration of amphetamine induced rotation behavior and will show improvements in tests of forelimb use and akinesia PUBLIC HEALTH RELEVANCE Recently it has been shown that embryonic stem cell derived dopaminergic cells that co express FOXA TH and Nurr are able to efficiently engraft in vivo and promote complete restoration of function in animal models of Parkinsonandapos s disease PD suggesting that the past failures of successful engraftment of DA cells were due to incomplete specification rather than a specific vulnerability of the cells Recently using chemical genetics approach for cell reprogramming we have been able to produce dopaminergic cells with similar gene expression profile from human bone marrow derived mesenchymal stem cells The goal of the proposed Phase I studies is optimization of this safe easy and cost effective cell reprogramming technology to increase the efficiency of the production of these engraftable midbrain dopaminergic neurons and elucidation of their therapeutic effect in an animal models of PD