Increase production of canna germplasm using multiplex PCR screening and antiviral treatments for rhizome sanitization

Horn Farms Inc needs to routinely produce sufficient annual seed stocks to support commercial production of key canna varieties. Thus the greenhouse diagnostic and sanitization program is necessary to meet production goals. Accurate visual disease assessment is carried out at 2 and 4 weeks of growth (instead of 10 and 16 weeks). Genus specific PCR primers were developed which detect all viruses in 3 tests. Plants are maintained in the greenhouse for 4-6 weeks and then moved to the fields at Horn Farms for use as planting stocks. The greenhouse is immediately replanted and the workflow repeats.Thus, the goal of this SBIR is to shift production to a higher level to meet market demands. Germplasm screening and quality control is essential for consumer confidence in the products sold by Horn Canna Farms and the goal is to have sufficient inventory of selected varities that Horn Canna Farms can use this program in promotional advertising and sales. This SBIR is essential to develop a solid infrastructure and revenue drivers that are vital for the longevity of Horn Farms.