A Tissue Implantable Microbiosensor

DESCRIPTION provided by applicant Abstract In this Phase II SBIR we will commercialize tissue implantable microbiosensors to measure glucose lactate and oxygen for use in freely moving animals We will also develop and commercialize a sensor array composed of a glucose biosensor a lactate biosensor and an oxygen sensor Biosensors provide high temporal resolution and low analyte consumption when compared to microdialysis Current state of the art biosensors O D m have proven value in the measurement of a range of analytes in the brains of freely moving rodents Reduction of biosensor diameter will result in considerably less damage to brain tissue and improved anatomical accuracy over current microdialysis and biosensor techniques Pinnacle will lead this proposal and work in conjunction with an interdisciplinary consortium of three leading scientists at the University of Kansas Professor Shenqiang Ren possesses extensive experience in materials science and nanofabrication techniques Professor Mark Richter is skilled in protein engineering and Professor George Wilson brings over years of experience in biosensor development to the project The facilities and equipment available at Pinnacle and the various University of Kansas laboratories will provide the resources required to successfully complete this project Innovative aspects of this proposal include new technologies in biosensor manufacturing such as nanoetching and electrophoretically manipulated enzyme immobilized nanoparticles and the ability to routinely and precisely deposit picoliters of substrate into a defined well In Phase I our team made several significant advancements in the development of a prototype m glucose microbiosensor We produced functional prototypes that measured glucose in a linear fashion to at least mM rejected ascorbate and urate and efficiently recycled O at the electrode surface These preliminary results form the foundation for the Phase II proposal These implantable m microbiosensors have the potential to significantly increase the understanding of the mechanisms behind drug neuron interactions This will profoundly change the drug discovery landscape and improve drug development efficiencies for pharmaceutical and biotech companies In addition increased spatial resolution and decreased inflammation responses will allow researchers to identify new neural processes leading to new understandings approaches and solutions for common maladies Biosensor sales in FY were $ billion with of the sales due to human glucose sensors Pinnacle is an established manufacturer of biosensors and is well positioned to introduce this new class of biosensors to a broad market PUBLIC HEALTH RELEVANCE In the United States over disorders of the brain and nervous system result in more hospitalizations than for any other disease group including heart disease and cancer The quality of life and economic cost of brain and nervous system related disorders is staggering These disorders disrupt the lives of more than million Americans each year and costs exceed $ billion