High-throughput Identification of Membrane Protein MAbs

DESCRIPTION provided by applicant Membrane proteins control the flow of information nutrients and signals through the cell membrane and are the targets for more than of FDA approved drugs Monoclonal antibodies MAbs that target membrane proteins can be exceptionally useful in research diagnostic and therapeutic applications but for most membrane proteins there are no MAbs that recognize the native protein on the cell surface The need for such MAbs has been recognized by industry and the NIH but efforts to identify such MAbs are limited by the difficulty in expressing and purifying membrane proteins in exogenous systems and by conventional MAb isolation strategies that typically focus on one target at a time A novel approach to identify membrane protein MAbs in a high throughput manner is needed to derive MAbs against the entire human membrane proteome Here we propose a platform technology that can be used to rapidly isolate MAbs against structurally intact membrane proteins for therapeutic development diagnostics and biomedical research PUBLIC HEALTH RELEVANCE This proposal will contribute to public health and the cure of human disease by identifying MAbs against native membrane proteins enabling their development for therapeutics diagnostics and biomedical research