Development of a fiber-based, holographic coherent anti-Stokes Raman scattering microscope for label-free imaging

DESCRIPTION provided by applicant Coherent Raman microscopy has emerged as an important label free imaging modality which can enable long duration non invasive imaging of cells and tissues However current coherent Raman systems require scanning to obtain three dimensional images hindering studies of rapid dynamic biological processes Additionally coherent Raman imaging systems usually rely on complex and expensive laser sources which limit their accessibility In this Phase I SBIR study Atoptix LLC proposes to develop a fiber based holographic coherent anti Stokes Raman scattering CARS microscope to overcome these limitations of scanning and system complexity The key innovation CARS holography merges the unique capabilities of CARS spectroscopy and holographic imaging by recording of a digital CARS hologram which captures both the amplitude and the phase of an anti Stokes image signal CARS holography can thus perform label free D imaging without scanning and have an improved sensitivity over conventional CARS due to its homodyne detection nature Furthermore the proposed system will use a robust turn key fiber laser based excitation system which overcomes the complexities and high costs associated with existing coherent Raman systems To perform this work Atoptix will collaborate with Penn State University researchers who are the inventors of CARS holography Specific Aim is to develop a synchronized two color ultrafast fiber laser system that will provide the CARS pump probe and Stokes excitation sources Specific Aim is to develop the prototype holographic CARS microscope The system will be characterized and optimized to assess performance and capability for Phase II development of an advanced prototype system PUBLIC HEALTH RELEVANCE Coherent Raman microscopes have emerged as important label free imaging tools for studying cells and tissues However current systems are reliant on scanning limiting the ability to monitor fast processes and use complex and expensive laser systems which reduces accessibility of such instruments This program proposes to develop a fiber laser based holographic coherent anti Stokes Raman scattering microscope that overcomes these obstacles