GTS SST-OPS: DNA Origami-based Bio-scavengers for Nerve Agent Sequestration

The objective of this proposal is to develop a robust and modular DNA single stranded tile (SST) technology that can be used to scaffold organophosphate scavengers (OPS). General Technology System"s (GTS) solution, termed SST-OPS, will is based off a well characterized and low-risk platform technology that can be readily used to construct molecular structures with high stability and high avidity towards organophosphates; SSTs have already been shown to be stable to at least 70C, capable spatially position scaffolded molecules with a 2.5 nanometer precision, and is based entirely on non-immunogenic DNA material,. Here, we propose to study how to optimize SST-OPS structures to best scavenge model OP, so that GTS may deliver an OP scavenging system that can meet and exceed outlined Phase I requirements. In addition for optimal scavenging performance, GTS will also provide formulations for SST-OPS structures (such as highest in vivo stability, OPS strand modification for varied OP targets, and modification for OP scavenger sensing) that are best suited for meeting potential Phase II and Phase III requirements.