RASL-Seq CTC Assay

DESCRIPTION provided by applicant This project will demonstrate the feasibility of identifying circulating tumor cells CTCs that have undergone an epithelial mesenchymal transition EMT by profiling a gene expression signature using a targeted sequencing platform the RASL Seq assay The presence of CTCs that have undergone EMT is prognostic of poor long term survival The gene profile will also include genes for identifying and counting EpCAM and EpCAM CTCs and for identifying the expression of targets for therapeutic agents Thus the RASL Seq CTC Profiling signature assay will deliver the EpCAM counts that the current Veridex CellSearch R assay does but provide significant additional utility by identifying EpCAM cells that have undergone EMT not assessed by CellSearch and which put the patient at risk as well as provide the oncologist with an indication of what therapy these cells may be responsive to overall significantly improving patient care and survival outcome The EMT and risk signature used in this proposal is the subject of patents filed by MD Anderson BioSpyder which has separately licensed the RASL Seq technology from UCSD will demonstrate its feasibility in Phase I determining if gene expression profiling of individual CTCs is required or if pools of e g EpCAM and EpCAM CTCs can be used for testing Initial development and validation will be with cell lines then a set of patient samples and finally a set of patient samples CellSearch results for these patients will be compared to the RASL Seq assay results to determine the positive predictive value of the RASL Seq test for Epcam CTCs The results of this program will demonstrate that the RASL Seq profiling assay has broad applicability not just to CTC analysis but to analysis of biopsies and for use as a companion diagnostic PUBLIC HEALTH RELEVANCE This project will demonstrate the feasibility of RASL Seq prognostic assays based on the embryonic EMT gene expression profiling of circulating tumor cells Specifically the feasibility for a breast cancer assay that is prognostic of relapse free survival and suggest response to therapy for patients at risk of developing relapse thus improving patient outcome The assay will count EpCAM CTCs and also identify whether CTCs including EpCAM CTCs that are not monitored using the current CellSearch assay have undergone EMT and therefore have metastatic potential with poor clinical outcome