Automated High-throughput Platform for Tissue Homogenization and RNA Extraction

DESCRIPTION provided by applicant The overall market size for nucleic acid sample preparation for research and the four applied markets of food testing cosmetics veterinary medicine and molecular diagnostics total $ billion in and with a moderate CAGR of is projected to $ billion in Extraction of nucleic acids from tissue has become a crowded field yet sample preparation and isolation of RNA still relies heavily on manual steps Providing a rapid automated system for tissue preparation and RNA isolation is an unmet need We have a unique cell and tissue lysing system the microHomogenizer mH which is a low cost disposable and rapidly effective homogenizing unit It consists of a disposable motor and vane that attaches to a pipette tip This facilitates ready adaptation to any robotic liquid handin system for automation and multiplexing We are poised to multiplex eight samples at a time for homogenization in a microtiter format thereby enabling automation of the subsequent RNA extraction protocol for samples at a time The extraction system of mono dispersed beads packed into small columns facilitates extraction protocols that transcend the need of a centrifuge This enables rapid automation of the extraction protocols in a microtiter format up samples at time PUBLIC HEALTH RELEVANCE We have a low cost disposable system of tissue homogenization the microHomogenizer and disposable columns for RNA extraction RNAExpressandquot columns that will be adapted to liquid handler platforms enabling multiplexing in a microtiter format and walk away automation