MIST System for Simultaneous Parallel Intracellular Delivery & Transfection

DESCRIPTION provided by applicant Alternative nanomaterial and gene transfer solutions are needed to access the potential held in intracellular imaging biopharmaceuticals gene therapy and stem cell research Delivery of small and macromolecules including nucleic acids drug molecules imaging agents peptides antibodies and enzymes into cells is critical to realizing their full potential in a rnge of research and therapeutic applications Unfortunately efficient intracellular delivery and transfection continue to elude researchers working with primary and stem cells in emerging application areas OpenCellandapos s vision is to create new intracellular delivery tools that enable researchers in emerging application areas to work with andquot difficult to transfectandquot cells for studies that are not currently possible Our core technology the Electrosonic Actuation Microarray EAM offers researchers a cost effective and simple to use solution to uniform and scalable treatment of entire cell populations on a cell by cell basis Multple Integrated Sample Treatment MIST the subject of this project proposal addresses cost and performance deficiencies of available methods by incorporating the innovative features of the EAM technology into a multichannel format In addition MIST uniquely streamlines protocol optimization and large scale screening by evaluating treatment outcomes for multiple experimental conditions including operating parameters and sample compositions in parallel During the Phase I project a MIST prototype achieved biomolecule delivery performance equal to that of our single sample STEAM system in a channel format without cross contamination between channels We have recently demonstrated advantages of our core technology for large molecule delivery including in difficult to transfect primary monocytes and for improving transfection outcomes with combined mode treatment It is anticipated that these advantages will carry over to the production MIST system The primary objectives of this SBIR Phase II project are design manufacture and characterization o a production ready MIST instrument To achieve these objectives MIST systems with stand alone electronics and disposable multichannel cartridges for mechanoporation and DNA electrophoresis will be developed and transfection protocol optimization will be demonstrated along with a comparison of relevant assessment criteria with our single sample STEAM system and competing technologies PUBLIC HEALTH RELEVANCE The lack of successful commercial transfection technologies is holding back the potential held in intracellular imaging biopharmaceuticals gene therapy and stem cell research Multiple Integrated Sample Treatment MIST addresses the current need for alternative gene transfer solutions that effectively treat difficult to transfect cells e g primary progenitor and stem cells MIST offers researchers a high throughput tool for uniform and scalable treatment of entire cell populations with control at the level of a single cell and with the ability to treat multiple diferent samples simultaneously