Rapid, Low-Cost, and High-Fidelity DNA Synthesis and Assembly Techniques

We propose to build and demonstrate a highly parallelized automated DNA synthesis and assembly pipeline capable of producing 100% accurate DNA fragments up to 30 kb within 7 days of receiving DNA sequence, at a cost of less than $0.04/bp, and with a daily capacity of greater than 1Mb of synthetic DNA. The outcome of this work will be Synthetic Genomics Inc. (SGI) as a provider of synthetic DNA at a drastically lower cost and more rapid turnaround time by more than an order of magnitude compared to what is currently available. This would significantly enable and accelerate development of new products such as biofuels, biochemicals, and vaccines in academic, commercial, and government facilities.