Nanotrap Enabled Dried Biofluid Collection Paper

Ceres will integrate the proven Nanotrap platform into three distinct Nanotrap-enabled biofluid collection and preservation collection tools. These collection tools build upon the current sample processing platform by providing three unique biofluid collection, processing and preservation solutions, each containing the Ceres patented and specifically optimized Nanotrap particles and affinity baits. The Nanotrap-enabled collection tools will consist of: 1) dried biofluid collection paper substrates for low volume blood samples having proven feasibility demonstrated during Phase I of this SBIR program; 2) urine collection cups containing a new version of magnetic Nanotrap particles that separate targeted analytes from solution allowing the analytes to be stored and shipped within the magnetic lid; and 3) blood collection tubes for larger volume blood collection, providing the ability to immediately collect and protect low-abundance clinical analytes from samples stored or transported at ambient temperature. The purpose of developing this toolkit is to not only provide a method for improved sample preservation of low volume biofluid samples, but to also take advantage of all the key feature of the Nanotrap particles, which allow for enrichment of low-abundance analytes, exclusion of high-abundance proteins, and the ability to capture and protect analytes from a wide range of biofluid samples.