High-capacity and Cost-effective Manufacture of Chloroperoxidase

The low cost chloroperoxidase can be potentially applicable to the inactivation of chemical agents due to its exceptional stability, broad substrate profile, and high catalytic efficiency. However, the high cost of producing large amounts of CPO is a major obstacle precluding the formulation and field deployment of this enzyme for chemical agent decontamination. Infoscitex Corporation (IST) and University of Massachusetts at Lowell (UML) propose a technology for high yield commercial production of CPO by an improved strain of filamentous fungus Caldariomyces fumago. The high yields of the enzyme production by C. fumago will be achieved via an advanced method of strain improvement by its directed evolution for target resistance established at Infoscitex. The high activity and stability of the resultant product will be assured by use of the most advanced technology for CPO isolation and purification. The new CPO producing strain will be coupled with state of the art protein production and purification capacity to achieve the required kilogram-scale manufacturing capabilities.