Multi-functional Synthetic Co-polypeptides as Hemostatic Agents

The objective of this proposal is to optimize a synthetic co-polypeptide formulation for the treatment of traumatic wounds. We employ a robust synthetic platform to access diverse functional co-polypeptides formulated as nanoemulsions, vesicles, micelles and optically clear hydrogels. Prior studies have demonstrated biocompatibility and wound healing with synthetic co-polypeptide hydrogels in murine models. Preliminary coagulation screens of polypeptide preparations (varying size and charge) showed wide differences in plasma gel formation and thromboplastin induced plasma clotting times. We propose to design and optimize hemostatic elements into our synthetic co-polypeptide preparations. Initially, we will test changes in primary structure and concentrations of co-polypeptides. Efforts focus on charge distribution (to enhance coagulation), high polypeptide content (to absorb water), hydrogel formulation (to provide matrix-like structure) and combinations thereof. Moreover, our synthetic co-polypeptides are compatible with active pharmaceutical agents, including various hemostatic materials. For commercialization of the hemostatic medical aid, we will seek partners with experience selling through both military and private sector channels. We believe that the distinct advantage of our approach -independent and orthogonal modification of structure-to-function relationships- will support the design of a more effective medical aid for the treatment of major trauma and burn injuries.