A Novel Electrokinetics-Driven, Integrated Microfluidic Cartridge for Sample Preparation from Complex Matrices

Sample preparation has been recognized as the single most important challenge to be faced in the development of detect-to-warn (DTW) systems (NRC, 2005). Available commercial sample preparation technologies are expensive, slow (30 min to 2 hours) and require trained laboratory technicians for operation. Addressing this need, we propose to design, fabricate, demonstrate, and validate a novel integrated microfluidic cartridge exploiting advanced electrokinetics for preparation of complex liquid samples for genomic analysis. The Phase I study successfully demonstrated three critical elements: (1) sorting of bacterial cells (2) electric field driven microbial lysis, and (3) DNA capture and elution in a microfluidic extraction chamber. Physical prototypes of the components were fabricated and experimentally demonstrated. During Phase II, these components will be optimized and integrated along with a novel pre-concentrator to develop an integrated microfluidics sample preparation card. The integrated cartridge will be validated in challenge situations mixing simulants and threat agents with real-world contaminants. Proprietary design tools will be used to guide component development and layout optimization. An experienced, multi-disciplinary team with expertise in microfluidics engineering, microfabrication, and agent testing has been assembled.