Development of a multiplexed point-of-care assay for the detection of infectious agents causing fever in deployed soldiers.

This objective of this study is to develop a robust, field-grade, screening assay for the detection of fever-causing pathogens including Plasmodium sp., Salmonella, Rickettsial sp., Flaviviruses and Leptospira. The test will utilize lateral flow (dipstick) chromatography incorporated into a unique, multiplex, cassette that enables the simultaneous screening of multiple analytes. The proposed immunochromatographic assay would be a very useful rapid screening test to assess individuals presenting with fevers of likely infectious etiology. In this Phase 1 study we shall evaluate the ability of a panel of antigens, derived from the families of pathogens listed above, to function as the basis of a serological detection system. We will then construct a prototype assay that will be evaluated against sera from individuals with confirmed infections. Further development of the assay would take place in a Phase 2 proposal.