Nanotechnology-based System for the Detection and Photodynamic Neutralization of BW Agents

Our goal is to detect and eliminate BW agents rapidly. In this project, we will develop functionalized nanoparticle photodynamic agents that can be bioconjugated with monoclonal antibodies to rapidly detect and kill viral infections. During Phase I, we will concentrate on the design and fabrication of handheld prototype detector that can be used as a sentry sensor for virus, e.g. RSV, detection and elimination. The detection and elimination functions of the nanoparticles will be tested separately. In the Phase I option we will conduct some pilot studies for Brucella detection and elimination. Once the proof-of-concept is demonstrated for BW detection and elimination, in Phase II we will combine the detection and elimination functions and focus on the applications of these bi-functional nanoparticle agents for detection and elimination of Brucella that may be present following a BW agent release in open spaces. Phase I Objectives: 1) Synthesize CdTe, TiO2 and ZnO luminescent nanoparticles, 2) Bioconjugate anti-RSV F protein monoclonal antibody (clone 131-2A) to nanoparticles, 3) Design and fabricate a hand-held prototype detector for RSV and Brucella (based on two sizes of CdTe nanoparticles), 4) Test biocongugated TiO2 nanoparticles for RSV elimination.