Dipstick Assay for Field Detection of Leishmania Parasites using Quantum Dots

A PCR based assay has been developed to detect Leishmania parasites, but PCR requires sophisticated laboratory instruments and trained personnel to interpret the data. The parasite can also be identified in infected sand flies by skillful dissection and microscopic examination but this is subjective and not appropriate for field testing. Antibodies are available that can detect Leishmania parasites and a standard ELISA could be used for detection, but this like the other assays requires trained personnel and sophisticated laboratory instruments. To overcome these shortcomings, Agave BioSystems proposes to develop a dipstick assay to provide a practical tool for assessment of Leishmania infected sand flies in the field. Our novel dipstick assay would use new fluorescent quantum dot technology for detection. This quantum dot assay would be considerably more specific, sensitive, and stable making it superior to standard colorimetric dipstick assays.