A Handheld Monitoring System For Biomarkers Of Response From Blood

The goal of this SBIR Phase I effort is to demonstrate proof-of-concept for a functional handheld system to monitor biomarkers or response. Specifically, during the Phase I effort we will demonstrate a disposable biochip, which can be used as a part of the handheld system, for simultaneous detection of acetylcholinesterase inhibition and Metallothionein (MT1 & MT2) concentration using immunoassay techniques. The biochip will be designed to measure these biomarkers from whole blood samples. The disposable biochip will be fabricated using BioMEMS technology and will include integrated microneedle for minimally invasive blood sampling. It is anticipated that the biochip (approx. 1.5"x2.5"x0.25") can be used with ~ a scientific calculator sized, handheld analyzer (developed in Phase II) for point-of-care biomonitoring. The research team includes members who are renowned experts in their area, with specific backgrounds in BioMEMS device development (at Siloam Biosciences) and identification/detection of biomarkers of exposure (at Center of Environmental Genetics, University of Cincinnati). The ultimate goal of this effort is to develop at least two different disposable biochips to work with a handheld analyzer. The two biochips will each be designed to detect multiple biomarkers of response (panel) elicited from organophosphates/carbamtes and organic industrial chemicals.