Metabolomic Evaluation of Combat Personnel for Optimum Fitness and Performance

This Phase I proposal for evaluation of combat personnel for optimum fitness and performance will be based on measuring lipid and amino acid biomarkers prior to and during the performance of ultra-marathon participants. Plasma or serum samples will be collected before individuals participate in the marathon and at various times during the race. The specific aims are to analyze and profile structural changes in plasma metabolites that may indicate tissue or metabolic changes and to seek evidence of physiological stress during strenuous physical activity by measurement of eicosanoids, which are produced by macrophages and that can indicate possible compromise to the immune system. This study will be related to the research topic by developing analytical tools to identify biomarkers of physical readiness, and markers that will be predictive of potential stress damage and damage that occurs in individual soldiers during times of physical stress. Based on plasma metabolite profiles, Phase II research would seek nutritional means to optimize soldier physiological performance under stress in the field.