A Novel, Microfabricated, Electro-Immuno, Integrated Sensor-Sampler for Bioagent Collection and Detection

We propose to develop and demonstrate a compact, low cost/power, high-sensitivity, integrated sensor-sampler that provides vastly superior performance while concomitantly achieving drastic size/weight reduction. The overall device is expected to be of shoebox size, to operate with AC/DC power, to generate 90% capture of targeted particulates in liquid medium. Next, the advanced electrokinetic separation and bead-based immunoassay will be optimized and integrated with Micronics' state-of-the-art MicrocytometerO cartridge. In addition, this extremely sophisticated cartridge will feature on-chip reagent storage, lysing of cells and is fully integrated with a miniaturized, portable flow cytometer. The air-sampler will be seamlessly interfaced with this microfluidic detection system. Waste will be contained on-chip for safe disposal and minimal user training will be required. Detailed performance assessment with bio-simulants and HVAC pollutants will be carried out. Further ensuring success, CFDRC has assembled an experienced, multidisciplinary team, with expertise in sampler design, microfluidics, biosensing, MEMS/microfabrication and system integration.