Large Throughput Multiplex Analysis of Gene Expression

We will develop resources for large-throughput analysis of gene expression. The complement of genesexpressed in a given cell varies during development, in disease states and in response to hormonal sgene expression are the basis of cancer. This project will show the feasibility of "Multiplex Profillarge-throughput strategy for characterizing changes in gene expression and for retrieving the variaperform MP on gene transcripts synthesized by two methods targeted to open reading frames and 5' regdevelop conditions for multiplex electrophoresis of PCR products and for hybridization and retrievaltranscripts with "signature" oligonucleotides derived from conserved protein motifs. MP will be appland normal respiratory epithelium cell lines using signature oligos for G proteins. The differentialcould become molecular markers for these tumors.