SBIR/STTR Research Award

Mouse Chromosome-Specific Painting Probes

Award identifier b529f4b3f95ad9ea8a9d31793de440839e7de072f8a2055e85288bba7d4471c3Awarding agency Department of Health and Human Services

Award amount

$735,111

Program and phase

SBIR · Phase II

Award year

1995

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Award details

Awarding agency
Department of Health and Human Services
Branch
Not reported
Recipient company
APPLIED GENETICS LABORATORIES
Proposal award date
Not reported
Contract end date
Not reported
UEI
Not reported
Solicitation number
Not reported
Topic code
Not reported

Research abstract

We have described a new technology called "preparative in situ hybridization (Prep-I.S.H.), which is capable of isolating a set of cDNA or genomic clones according to their chromosome location. The technology takes advantage of in situ hybridization of cloned DNAs to metaphase chromosome spreads, chromosome microdissection, and the polymerase chain reaction (PCR). We will use this technology to develop a set of chromosome-specific painting probes for the mouse. The demonstration case, a chromosome 11-specific probe, will be used to paint the mitotic chromosome 11s derived from the mouse lymphoma assay (MLA).

Technology keywords

Not reported

Agency intelligence

Department of Health and Human Services

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