SBIR/STTR Research Award

TRANSLOCATION ASSAY BY PCR AMPLIFICATION IN SITU

Award identifier e84e5608c2f0060d468cd079ebd7a2149081b4344d61e338b9cde8da8f104549Awarding agency Department of Health and Human Services

Award amount

$48,557

Program and phase

SBIR · Phase I

Award year

1992

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Award details

Awarding agency
Department of Health and Human Services
Branch
Not reported
Recipient company
ONCOGENE SCIENCE, INC.
Proposal award date
Not reported
Contract end date
Not reported
UEI
Not reported
Solicitation number
Not reported
Topic code
Not reported

Research abstract

POLYMERASE CHAIN REACTION (PCR) AMPLIFICATION AND DETECTION IN SITY HAS BEEN DESCRIBED FOR THE HUMAN PAPILLOMAVIRUS (HPV 16, 1) AND VISNA VIRUS (2). USE OF PCR TECHNIQUES IS EXTREMELY POWERFUL YET HAS SOME DISADVANTAGES. PCR AMPLIFICATION IN SITU CIRCUMVENTS SOME OF THESE AND ALSO ALLOWS CORRELATION OF CELL MORPHOLOGY WITH MRNA EXPRESSION OR PRESENCE OF A TRANSLOCATION. IN PHASE I, METHODS OF PCR AMPLIFICATION IN SITY FOR CANCER RESEARCH, USING FORMALIN FIXED, PARAFFIN EMBEDDED CELL LINES WILL BE OPTIMIZED. THESE TARGETS WILL BE THE T(14; 18) BC12 (MBR)/IGH TRANSLOCATION USING SU-DHL-6 CELLS, AND RNA-PCR IN SITU FOR THE T(9; 22) BCR/ABL TRANSLOCATION, USING K562 CELLS. THE HL-60 CELL LINE WILL BE USED AS A NEGATIVE CONTROL FOR MIXING EXPERIMENTS. IN PHASE II AND III, ASSAYS FOR THE T(14; 18) BC12 (MCR)/IGH T(9; 22) BCR/ABL (ALL), T(1; 19) E2A/PRL-PBX, T(15; 17)MYL/RARA, AND T (6; 9) DEK/CAN TRANSLOCATION WILL BE DEVELOPED AND COMMERCIALIZED.

Technology keywords

Not reported

Agency intelligence

Department of Health and Human Services

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