SBIR/STTR Research Award
Protein Bound RNA Sequencing
Award amount
$225,000Program and phase
STTR · Phase IAward year
2014Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- National Institutes of Health
- Recipient company
- BIOO SCIENTIFIC CORPORATION
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Z24GLNTRPBT5
- Solicitation number
- PA13-088
- Topic code
- NHGRI
Research abstract
DESCRIPTION (provided by applicant): Protein-RNA interactions make a profound contribution to the regulatory logic of post- transcriptional control of gene expression. Tools to decipher this intricate layer of molecular interactions on a global scale are coming to the forefront of molecular biology and genomics. The most wide spread methodology to examine interactions of RNA binding proteins (RBPs) with their targets is an IP-microarray plagued by several experimental pitfalls. The cross-linking immunoprecipitation (CLIP) method overcomes many of these pitfalls while facilitating identification of RNA targets as well as the in situ bindng sites. Developing a novel tool to decipher this intricate layer of molecular interaction is a focusof this proposal. During development, we will identify noise arising from clonal reads and improve the sensitivity of the procedure. Analytical techniques will be developed to allow modeling of ligation efficiency and estimate clonal biases accurately. The ultimate goal of
Technology keywords
Not reported
Other awards by this recipient
Verified by the same UEI. These are other public SBIR/STTR award pages, not a merged company record.
- Sequencing of Epigenetic Modifications from Single Cells
- TARGETED THERAPEUTIC ANTIBODY MOLECULES AGAINST VIRAL ENCEPHALITIS
- Digital Gene Expression Analysis by 3’ End Sequencing
- Burkholderia antibody molecules for therapeutics and diagnostics
- SBIR Phase II: High-throughput Small RNA Sequencing
Awards with the same topic code
Verified by an exact topic code match. This is not a text-similarity match.
- Platform to support clinical variant interpretation through probabilistic assessment of functional evidence
- Innovations to expand and accelerate the commercial impact of DNA modifications
- High-throughput Direct Sequencing and Quantitative Mapping of RNA Modifications using Mass Spectrometry.
- Super-resolution single-molecule sequencing by observing the dynamic hybridization of a repertoire of oligonucleotides to DNA targets
- Exhaustive de novo sequencing of every RNA in a sample by a layer-by-layer mass spectrometry ladder intensity approach.
Research funding history
26 SBIR/STTR records share this UEI (2007–2016). Reported award amounts total $7,244,135. Reported amounts are not federal obligations.
Phases: Phase I, Phase II
Agencies: Department of Health and Human Services, National Science Foundation, Department of Defense, Department of Agriculture