Ecs--Electrophoretic Protein Crystallization System

Determination of detailed atomic structures of proteins and DNA has offered a wealth of informationworking of biological systems. Macromolecular crystallography has provided the vast majority of infostructures of viruses, proteins (including those of medical significance), and nucleic acids. Yet, tmacromolecule remains a trial-and-error process which often requires long periods of time and a largOur goal is to design and test a prototype protein crystal reaction chamber which will offer a newmacromolecules. In our device, a voltage permeated membrane will keep protein and precipitant solutielectric field is applied to the system. At that time, precipitant buffer will enter the protein chacrystallization. Some of the features of our system include electrophoretic concentration of proteincontrol of pH, temperature, mixing rates, and precipitant concentration during the experiment; rapidnucleation to growth conditions; re-use of protein sample; ease of use; reproducibility. Our new deva better understanding of macromolecular crystallization processes as well as to provide a more rapito the crystallization of macromolecules.