Development of a High-Throughput IVF System for Swine

Polyspermy remains the primary limiting factor for successful porcine embryo production in vitro. It has recently been demonstrated that fertilization of porcine oocytes performed in a microfluidic device led to a significant reduction in polyspermy over conventional IVF, while retaining overall penetration rates. The purpose of this study is to determine the mechanism by which the microchannel environment yields increased rates of monospermic fertilization compared to conventional methods of porcine IVF.