Hematopoietic Stem Cell Enrichment by Negative Selection

Stem cells are the agents of hematopoietic reconstitution in bone marrow transplantation and are atherapy. This proposal is to facilitate human stem cell research by providing a rapid, high yield syrecovering stem cells from bone marrow, cord blood or peripheral blood sources. This system consistsof specific sequential depletions of non-stem cell components using an erythrocyte precipitation bufimmunoaffinity column. This system is capable of processing large numbers of cells to produce high lfor CD34+ cells. Preliminary results with both bone marrow and cord blood using this method have demfold increase in stem cell concentration and >70% recovery of total CFU activity. The initial focusoptimize the procedure for enrichment of stem cells from different sources and determine the recovercells. This study will lead to the development of a product intended for the basic and clinical reseform the basis for a phase II submission intended towards development of this methodology as a closedevise.