MULTIPLE DRUG DETECTION IN FINGER PRICK BLOOD

WE WILL DEVELOP AND DEMONSTRATE THE FEASIBILITY OF AN ENZYMEIMMUNOASSAY FORMAT CAPABLE OF SIMULTANEOUSLY DETECTING AND IDENTIFYING ANY ONE OR MORE OF MULTIPLE DRUGS IN BLOOD FROM A FINGER PRICK. THE EVENTUAL GOAL IS TO DEVELOP A SCREENINGTEST DEVICE HAVING A BUILT-IN SAMPLER WHICH WOULD ALLOW THE USER TO TOUCH THE DEVICE ONTO A DROP OF BLOOD FROM A FINGER PRICK, TURN THE DEVICE OVER AND TAP IT TO INTRODUCE THE SAMPLE, PLACE THE DEVICE INTO A DEVELOPING SOLUTION, WAIT FOR A FEW MINUTES AND OBSERVE THE RESULTS. ANY DESIGNATED BANDS HAVING COLOR WOULD INDICATE THE PRESENCE OF THE CORRESPONDING DRUG. THE SPECIFIC GOAL OF THE PHASE I PROJECT IS TO DEVELOP THE TECHNIQUE, USING A SIMPLER APPARATUS, AND TO DEMONSTRATE THAT ANY COMBINATION OF COCAINE, MORPHINE AND PHENCYCLIDINE CAN BE IDENTIFIED IN A SMALL BLOOD SAMPLE, EACH AT 20 NANOGRAMS/ML OR GREATER CONCENTRATIONS. WE WILL DEVELOP AND DEMONSTRATE THE FEASIBILITY OF AN ENZYMEIMMUNOASSAY FORMAT CAPABLE OF SIMULTANEOUSLY DETECTING AND IDENTIFYING ANY ONE OR MORE OF MULTIPLE DRUGS IN BLOOD FROM A FINGER PRICK. THE EVENTUAL GOAL IS TO DEVELOP A SCREENINGTEST DEVICE HAVING A BUILT-IN SAMPLER WHICH WOULD ALLOW THE USER TO TOUCH THE DEVICE ONTO A DROP OF BLOOD FROM A FINGER PRICK, TURN THE DEVICE OVER AND TAP IT TO INTRODUCE THE SAMPLE, PLACE THE DEVICE INTO A DEVELOPING SOLUTION, WAIT FOR A FEW MINUTES AND OBSERVE THE RESULTS. ANY DESIGNATED BANDS HAVING COLOR WOULD INDICATE THE PRESENCE OF THE CORRESPONDING DRUG. THE SPECIFIC GOAL OF THE PHASE I PROJECT IS TO DEVELOP THE TECHNIQUE, USING A SIMPLER APPARATUS, AND TO DEMONSTRATE THAT ANY COMBINATION OF COCAINE, MORPHINE AND PHENCYCLIDINE CAN BE IDENTIFIED IN A SMALL BLOOD SAMPLE, EACH AT 20 NANOGRAMS/ML OR GREATER CONCENTRATIONS.