Automated Bisensors Operated Under Transient Mode

We will develop a reliable and sensitive single and multiple two-dimensional membrane oxygen electrode system as a potential screening system for chemotherapeutic agents. Our approach to developing this dissolved-gas measurement system is to build on an existing membrane electrode design and to use a microcomputer and an in-house developed software to overcome some of the problems associated with traditional membrane electrode technology. This non-invasive technology can be used in dynamic studies of basic cellular metabolism on factors affecting this metabolism. The technology can also be used in dynamic studies on cellular changes due to cytotoxic compounds, hormones, and other pharmacologically active drugs. Another use of this research is to examine the toxicity and safety of various chemical agents used in food. It can be applied in pre-clinical settings in testing different drug combinations and other combinations of chemotherapeutic regimen. In-vitro and in-vivo applications of this technology can also be extended to the biomedical area, e. g. monitoring glucose, pCO2, in blood , etc.