IGF OT IGF PROXIMITY DNA EXCHANGE IMAGING

A great challenge in microscopic imaging of biological and medical samples e g tissue sections is to achieve high sensitivity high specificity high multiplexing and high resolution preferably beyond the diffraction limit simultaneously This SBIR proposal addresses this challenge by developing a novel technology platform called Proximity DNA Exchange Imaging As a result of the studies proposed here a panel of imaging reagents three high value cancer related biomarkers phosphorylated AKT MET and CTNNB will be established Compared to previous and current proximity based immunoassay platforms Proximity DNA Exchange Imaging is highly advantageous with regard to ease of implementation versatility depth of multiplexed information and spatial resolution This technology will offer the research and medical community methods to investigate molecular details of biology with unprecedented precision and information content It also has broad applications in areas such as digital pathology and high content screening for drug discovery