SBIR/STTR Research Award
DIRECT MALDI-TOF ANALYSIS OF LCM-SELECTED TISSUE & TUMOR
Award amount
$100,000Program and phase
SBIR · Phase IAward year
2002Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- National Institutes of Health
- Recipient company
- BIOANALYTE, INC.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
DESCRIPTION (provided by applicant): In order to improve the quality of
differential cancer diagnostics and metastatic tumor origination using protein
and carbohydrate biomarker detection of patient biopsies, BioAnalyte Inc. and
collaborators at MGH and JHMI propose to develop laser capture microdissection
(LCM) as a sample preparation system for the biomolecular profiling capabilities of MALDI-ToF mass spectrometry. The work will first optimize
protocols for collecting cells from mammalian tissue for MALDI-ToF analysis.
For differential diagnostics we will analyze several LCM-selected cells from
the tumor collection at MGH representing a variety of cancers will be used to
determine the MALDI-ToF profiles of transformed and adjoining normal tissue.
For identification of a metastatic tumor with its primary, we will make
MALDI-ToF MS profiles of LCM-selected cells from metastatic cancers and compare
them to profiles of LCM-selected cells in the original tissue. New software
about written for general MALDI-ToF chemometric analysis of profiles WII be
implemented here to determine fingerprint regions of the mass spectra in each
of its applications. Hardware to optimize the coupling of LCM transfer caps to
MALDI-ToF ionization sources will be developed.
Technology keywords
Not reported