ENDOSCOPIC BIPOLAR SCISSORS

DESCRIPTION (Adapted from applicant's abstract): Endoscopic techniques are revolutionizing many surgical procedures and further expansion is inevitable. The essence of minimally invasive surgery is to operate in a dry field, avoiding major hemorrhage, which can force conversion of the endoscopic approach to a laparotomy. Electrosurgical devices fulfill the need for tissue cutting with simultaneous vascular coagulation in a cost- effective manner. However, monopolar systems can conduct current along unwanted pathways through the patient and cause tissue damage. Safety concerns and intensified by the limited field of view. Recently, bipolar scissors have been developed, which address the safety concerns. But, the scissors are limited by incompatibility with many generators, mechanical complexity, slow or ineffective coagulation, relatively high cost to manufacture, and loss of feel for the surgeon who is accustomed to conventional "metal to metal" cutting surfaces. To overcome these limitations, the PI has devised a new type of bipolar scissors, in which, each blade is a bipolar electrode. This design allows for multiple local current pathways, high current densities, and lower impedance. These scissors should work with most generators, have greater coagulation efficiency, and higher cutting speeds. In Phase I, the principal investigator proposes to fabricate, test, and compare the bipolar scissors with other monopolar and bipolar scissors in benchtop and in acute in vivo studies. $ = TOTAL AWARD AMTS & NOT LIMITED TO PORTION OF PROJECT RELATED TO SUBJECT OF SEARCH SUBPROJECT $ = TOTAL AWARD AMOUNT DIVIDED BY NUMBER OF SUBPROJECTS SOURCE: CRISP FORMAT F FY 97 LAST UPDATE 04-07-98 1QUERY 1536 ID SEARCH 06/01/98 PAGE 407 --PROJECT NUMBER......1 R43 HL56531-01A1 INVESTIGATOR NAME/ADDRESS FY 97 BOLES, TRUETT C IRG/INTRAMURAL UNIT..ZRG2 MOSAIC TECHNOLOGIES, INC AWARD AMOUNT......... $66,750 255 BROADWAY WINTER HILL, MA 02145 PERFORMING ORGANIZATION: MOSAIC TECHNOLOGIES, INC. TITLE ARC DETECTION FOR AMPLIFICATION-BASED GENETIC TYPING ABSTRACT: The overall objective of this proposal is to provide a fast and reliable automated method for amplification-based genetic typing of point mutations and allelic single-base changes. The proposed method called ARCTM detection, is designed for analysis of highly polymorphic DNA targets that can be amplified by the polymerase chain reaction (PCR). The method can simultaneously type hundreds of nucleotide positions using the amplified products from a single PCR reaction. The ARCTM process relies on polymerase extension of allele-specific primer pairs that have been immobilized on a solid support. ARCTM detection will provide faster and more reliable typing results than allele-specific hybridization, with less technician labor, and should have broad applications in HLA typing, diagnosis of genetic disease, infectious disease diagnostics, forensics, genetic mapping and mutation analysis. In phase I, the feasibility of the proposed method will be tested by constructing a prototype, twelve-assay, multiplex ARC detector for typing all HLA-DQA1 alleles using a single amplification reaction.