Rapid, Sensitive Sequencing of 2-D Gel Separated Proteins

This project is to develop a new technology to obtain sequence information on proteinsseparated by 2D-gel electrophoresis. This instrument will be capable of sequencing multiple samplessimultaneously, with greater sensitivity than that obtained with current technology. These features willgreatly increase the efficiency of sequencing the many proteins that may be present following the 2D-gelelectrophoretic separation of complex protein mixtures, for example for analysis of the proteins presentin a cellular lysate. The key components that distinguish the proposed instrument from commercialmodels are: utilization of piezoelectric droplet generators for the precise delivery of small volumes ofreagents; utilization of a two-of-flight mass spectrometer for detection of the sequencing products; and,the capacity for sequencing multiple samples simultaneously.