RNA Detector-Molecule Methodology for Microorganism Biodetectors

Current geopolitical strategies require effective warfare countermeasures to protect U.S. forces against biological and chemical threats. Today's molecular biology and immunological detection technologies cannot be used to produce automated biodetectors useable in the battlefield. New, improved detection technologies are needed to minimize the impact of chemical and biological weapons on Army personnel and provide a means to construct automated biodetectors that can identify a very few particles of any hazardous agent, regardless of interferant background. The final objective of the work proposed is to develop a highly sensitive RNA probe methodology to identify pathogenic bacteria and viruses. The methodology will underlay the foundation of RNA based tests that will be easy to perform as a simple enzymatic reaction at 37 degrees C under isothermal conditions in a single test tube format and can identify less than 100 target molecules in a specimen. Because of its extreme simplicity, the test could ultimately be incorporated into a small, portable, personal deviceuseable in battlefield environments without special training. This technology goes beyond normal evolutionary development approaches because it breaks the existing paradigms of the diagnostic industry by demonstrating the technical feasibility of detecting a non-nucleic acid target using a nucleic acid detector system.